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- W2382471335 abstract "Objective: To observe the expressive levels of calcitonin gene-related peptide(CGRP) in bone marrow mesenchymal stem cells(BMSCs) during osteoinduction, and in the remodeled bone of maxillary sinus floor augmentation.Methods: In vitro, the BMSCs were isolated from Beagle dogs and were classified into osteogenic-induced group and noninduced group. Expression of the CGRP was detected by Q-PCR and immunofluorescence in the different time-points. In vivo, both right and left maxillary sinus of three healthy adult dogs were divided into experimental group and control group randomly. BMSCs complexed with Bio-Oss granules were applied for canine maxillary sinus augmentation models, while Bio-Oss granules alone served as controls. Immunohistochemical analysis were performed after 12 weeks on these bone substitutes. Results: Q-PCR and immunofluorescence examinations demonstrated the expression of CGRP in osteogenicinduced group was increased in a time-dependent manner. After maxillary sinus floor elevation, the expressive levels of CGRP in experimental group were superior than that of control group(P0.05). CGRP was mainly distributed in the region where osteogenesis metabolism was active. Conclusion: The expression of CGRP was related to the progress of bone regeneration, and it can be regarded as an indicator of the activity of osteogenesis metabolism." @default.
- W2382471335 created "2016-06-24" @default.
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- W2382471335 date "2014-01-01" @default.
- W2382471335 modified "2023-09-23" @default.
- W2382471335 title "The Expression of Calcitonin Gene-related Peptide during Bone Formation" @default.
- W2382471335 hasPublicationYear "2014" @default.
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