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- W2382522949 abstract "Objective To compare the cell morphology,growth rate,differentiation capacity and the ability of regulating peripheral blood mononuclear cell(PBMC) to secrete IL-10 and IFN-γ by bone marrow mesenchymal stem cells(BMMSCs) which were isolated from immune thrombocytopenia(ITP) patients,healthy adults and umbilical cord mesenchymal stem cells(UC-MSCs).Methods The 5-6 generations of BMMSCs were isolated from ITP patients(n=16) and healthy adults(n=8) using density gradient centrifugation.The 5-6 generations of UC-MSCs were isolated from healthy fetuses′ umbilical cord using enzyme digestion.In the process of culture,the morphology of three kinds of MSCs were observed;the immunophenotype,adipogenic and osteogenic differentiation potential of them were identified;the reproductive activity of them was assessed by cell counting kit-8(CCK-8).Besides,three kinds of MSCs were inactivated by mitomycin C(MMC) and co-cultured with phytohaemagglutinin(PHA)-activated PBMC with the ratio of 1:40,3:40 and 9:40 respectively.Three days later,the culture supernatant was collected and the cytokines IL-10 and IFN-γ were measured by ELISA.Results Three kinds of MSCs had similar cell morphology,immunophenotype and differentiation potential.UC-MSCs showed the fastest growth rate;BMMSCs of ITP patients was the slowest one and the BMMSCs of healthy adults was between them.Three kinds of MSCs were co-cultured with different concentrations of PBMC.The results showed that the more propotions of MSCs,the more increase of IL-10(in BMMSCs of ITP patients group,P0.05;in BMMSCs of healthy adults and UC-MSCs groups,P0.01);conversely,the more propotions of MSCs,the more decrease of IFN-γ(in BMMSCs of ITP patients and healthy adults groups,P0.05;in UC-MSCs group,P0.01).There was no significant difference among three groups when co-cultured in the same conditions(P0.05).Conclusions Three kinds of MSCs had the same characteristics in regulating PBMC to secrete IL-10 and IFN-γ: promoting the secretion of IL-10 and inhibiting the secretion of IFN-γ levels by PBMC in a dose-dependent manner.There was no significant difference among MSCs from different origins in regulating PBMC to secrete IL-10 and IFN-γ." @default.
- W2382522949 created "2016-06-24" @default.
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- W2382522949 date "2012-01-01" @default.
- W2382522949 modified "2023-09-23" @default.
- W2382522949 title "The different immunomodulation of the mesenchymal stem cells from ITP patients bone marrow and other origins" @default.
- W2382522949 hasPublicationYear "2012" @default.
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