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- W2382534093 abstract "Objective To construct the human vascular endothelial growth factor 165 (VEGF165) expression vector express and purify VEGF165 protein in E. Coli XL-Blue for testing biological activity. Methods Total RNA isolated from human fetal liver tissue was used as a template. The entire coding region of the VEGF165 was amplified, purified and inserted into pET-32a (+) Vector to facilitate expression. The sequence of the inserted fragment was verified by PCR identification and DNA sequencing. Correct plasmids were transformed into E. Coli XL-Blue. The biological activity of VEGF165 was tested through the effect on proliferation of human umbilical vascular endothelial cell (HUVEC). Results After IPTG induction, the expression of VEGF165(His)6 was obtained. Western blot analysis showed that VEGF polyclone antibody specifically bound to 26 kD band. Pure recombinant VEGF165 protein was obtained by High-affinity His-bind Purification system, which has the biological founction of promoting proliferation. Conclusion We cloned VEGF165 to expression the biological activity recombinant protein in E. Coli XL-Blue, which facilitates the study of the biological function of VEGF165." @default.
- W2382534093 created "2016-06-24" @default.
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- W2382534093 date "2008-01-01" @default.
- W2382534093 modified "2023-09-26" @default.
- W2382534093 title "Expression and biological activity test of the VEGF_(165) gene" @default.
- W2382534093 hasPublicationYear "2008" @default.
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