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- W2382580702 abstract "Objective To investigate the neuronal differentiation of the neural stem cells derived from a human fetal brain after transplanted into young rats' brains.To study the possibility of cell-replacement therapy for children's brain disorders with neural stem cells.Methods The neural stem cell spheres were cultured out from the fetal brain tissues of 16 week gestation.The differentiation multipotency of the neurosphere was identified when cultured in children cerebrospinal fluid(CSF).The neurospheres cultured in vitro for fourteen days were injected into the lateral ventricles of young rats of 10 days old.The rats were killed at 4d,7d,and 14d post-transplantation.The brains were sectioned,and the special immuno-fluorescent assays were performed using anti-human neurofilament(anti-hNF) to show the location and morphology of graft neurons.Results Typical floating neurospheres were obtained,with the potency to differentiate into neurons,astrocytes and oligodendrocytes.The neuronal differentiation of grafts was detected with the mixture of three monoclonal antibodies against human neurofilament.Four days after transplantation,the positive staining cells within the granule cell layer of cerebral cortex were shown in the shape of granule cells,or within the pyramid cell layer in the shape of pyramid cells with long processes,and the interneuron-like cells also were watched.The Purkinje cells arranging in a monolayer row were detected in the cerebellum.At the different time points of 4d,7d,14d after injection,the graft cells were less and the processes were longer with the time extending while the location of grafts were the same.Conclusion The in vitro cultured neurosphere cells can migrated into brain tissues and differentiated into site-specific neurons in shape after transplanted into the lateral ventricles of young rats,suggesting that the environment of host brain tissue plays an important role in guiding the graft differentiation into neurons." @default.
- W2382580702 created "2016-06-24" @default.
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- W2382580702 date "2005-01-01" @default.
- W2382580702 modified "2023-09-26" @default.
- W2382580702 title "Site Specific Neuronal Differentiation of Human Neural Stem Cells in Host Rat Brains" @default.
- W2382580702 hasPublicationYear "2005" @default.
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