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- W23825914 abstract "Publisher Summary This chapter presents a study examining matrix phase organization in the adrenal chromaffin granule. The formation of chromogranin-containing phospholipid particles was carried out by application of a method primarily designed for the formation of unilayered phospholipid vesicles, that is, liposomes. The sonicated suspensions as well as the isolated particles were highly potentiated with respect to dopamine- β-hydroxylase by the presence of phospholipids in the preparations. The stimulating effect was proportional to increases in the lipid P /protein ratio in a manner which pointed to cooperative effects of the phospholipids on the enzymic protein. Thus, the phospholipids appeared to interact with the enzyme and thereby regulate the rate of enzyme activity, in keeping with the higher specific activity of dopamine-β-hydroxylase in the liposomal fraction of the lysis-resistant membrane phase. The artificial phospholipid-chromogranin particles exhibited a certain capacity for uptake of noradrenaline (NA) in presence of ATP and the uptake was 10 times higher than that observed for Ca 2+ , suggestive of a preference for the amine." @default.
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- W23825914 date "1973-01-01" @default.
- W23825914 modified "2023-09-26" @default.
- W23825914 title "MATRIX PHASE ORGANISATION IN THE ADRENAL CHROMAFFIN GRANULE" @default.
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- W23825914 doi "https://doi.org/10.1016/b978-0-08-017922-3.50085-x" @default.
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