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- W2382592753 abstract "The ability of in vitro maturation,fertilization and further development of bovine oocytes derived from interior ovary(IO-oocytes)was studied.The addition of FSH(10 μg·mL -1),hCG(20 μg·mL -1)and E 2(1 μg·mL -1)to maturation medium had a significant effect on the ability of fertilization and subsequent development of IO-oocytes(P0.01)and the similar effect was in the group of single addition of oestrous cow serum .The rate of cleavage and development of 68 cells was 34.21%36.80% and 23.68%24.53% of 2124 h culture after insemination respectively.It would reduced the rate of cleavage(P0.01)and development of 68 cells(P0.05)to increase or decrease the cultural time.The TALP method had the similar rate of cleavage and a higher developmental rate of 68 cells(P0.05)compared to BO method.Mechanical removal of cumulus cell prior to insemination significantly reduced the rate of cleavage and development of 68 cells(P0.05).The presence of cumulus cells or granulosa cell monolayers in maturation medium increased the developmental rate of 68 cells of the IO- oocytes after in vitro maturation and in vitro fertilization(IVM-IVF)(P0.05)." @default.
- W2382592753 created "2016-06-24" @default.
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- W2382592753 date "2003-01-01" @default.
- W2382592753 modified "2023-09-27" @default.
- W2382592753 title "In vitro maturation and fertilization of bovine oocytes from interior ovary" @default.
- W2382592753 hasPublicationYear "2003" @default.
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