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- W2382607416 abstract "To investigate the polymorphism of major histocompatibility complex ⅡDRB1(MHC-DRB1) in Wadi sheep,the second exon of this gene in 82 Wadi Sheep was amplified by nested PCR and the amplified products were characterized by RFLP technique using 2 restriction enzymes,ie,SacⅠandHaeⅢ.The results showed that the extensive polymorphisms of the exon2 of MHC-DRB1 were found at two loci digested with SacⅠandHaeⅢ,respectively,and these sites were controlled by two,five alleles,respectively.As some base mutations were found in MHC-DRB1locus,and according to the results in this paper,fifteen genetypes were found in Wadi sheep.The new allele MHC-DRB1.D was identified only in these Wadi sheep populations.The nucleotide and predicted amino acid sequence alignments of MHC-DRB1 alleles provided the evidence for considerable variation among the five alleles." @default.
- W2382607416 created "2016-06-24" @default.
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- W2382607416 date "2011-01-01" @default.
- W2382607416 modified "2023-09-25" @default.
- W2382607416 title "Polymorphism Analysis of MHC-DRB1 Gene in Wadi Sheep by PCR-RFLP" @default.
- W2382607416 hasPublicationYear "2011" @default.
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