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- W2382641816 abstract "Through preliminary petri-dish and following by shaking flask fermentation screening,a highest-yield amylase HYM-7 was isolated from samples in Bohai Sea area-Qinhuangdao,and it was primarily identified as Paenibacillus. amylolyticus. Its fermentation condition was studied through the single-factor and orthogonal experiment. The results demonstrated that after 48h it had the maximum amylase activity under 1% the vaccination quantity when 50mL broth in 250mL flask,And the optimal carbon and nitrogen source was corn flour and soybean flour respectively,Ca2+ and Mg2+ were beneficial to enzyme activity obviously. Through orthogonal experiment obtained the top-seeded formula for the media(g/L):corn flour 4,soybean flour 10,soluble starch 2,magnesium sulfate 0. 2 and calcium chloride 0. 4. Finally,the optimized fermentation medium was validated,and the total activity of amylase was 34. 62 U·mL-1,which was 60. 8% more than basic medium." @default.
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- W2382641816 date "2010-01-01" @default.
- W2382641816 modified "2023-09-25" @default.
- W2382641816 title "Fermentation conditions of marine strain HYM-7 producing cold-active amylase" @default.
- W2382641816 hasPublicationYear "2010" @default.
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