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- W2382679757 abstract "A pair of primers with restriction sites Kpn I and Xba I were designed and synthesized according to the gene sequence of Musca domestica larvae defensin gene Mdd I,then facilitated to amplify the full- length sequence of Mdd I by polymerase chain reaction(PCR).After cloning and sequencing,the eukaryotic expression plasmid pGAPZαA- Mdd I was constructed and then expressed in P.pastoris GS115,subsequently the antimicrobial activity of recombinant protein was analyzed.The result showed that the expressed protein of Mdd I was about 10.3 kDa,which possessed antibacterial activity against swine Streptococcus.Conclusively,the recombinant P.pastoris with gene Mdd I was successfully constructed,which was a basis for further researchers in biological and immunologic activities of eukaryotic expression product of Mdd I." @default.
- W2382679757 created "2016-06-24" @default.
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- W2382679757 date "2015-01-01" @default.
- W2382679757 modified "2023-09-23" @default.
- W2382679757 title "Expression of Musca domestica Larvae Defensin Gene Mdd I in P.pastoris and Identification of Its Antibacterial Activity" @default.
- W2382679757 hasPublicationYear "2015" @default.
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