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- W2382722018 abstract "【Objective】 To investigate the differential mRNA expression between intramuscular and subcutaneous adipocytes of pig. 【Method】 Conventional collagenase-based procedures were modified to harvest preadipocytes from longissimus dorsi muscle and subcutaneous adipose tissue of neonatal piglets (Large-White×Landrace) respectively, and the preadipocytes were then exposed to DMEM/F12 medium containing 5% new born bovine serum (NBS), 850 nmol·L-1 insulin (Ins) and 50 nmol·L-1 dexamethasone (Dex). The concentrations of intracellular triglyceride were measured by Oil Red O staining. The mRNA expression level of genes involved in adipogenesis, such as peroxisome proliferator-activated receptors γ (PPAR γ), CCAAT/enhancer binding protein β (C/EBP β), sterol regulatory element binding protein-1 (SREBP-1), acetyl-CoA carboxylase (ACC), stearoyl-CoA (Δ9) desaturase 1 (SCD-1), lipoprotein lipase (LPL), fatty acid translocase (FAT), and adipocyte fatty acid binding protein (aP2) in the two preadipocytes were compared by real-time PCR, so were those of cytokine receptors, such as activin receptor 2B (ACVR2B), interleukin-6 receptor (IL-6R), insulin-like growth factor-1 receptor (IGF-1R). 【Result】The results showed that intracellular triglyceride concentrations of intramuscular preadipocytes increased more dramatically than that of subcutaneous preadipocytesduring cell differentiation, paralleling with the higher mRNA expression levels of PPAR γ, SREBP-1, SCD-1, LPL, AP2 and FAT in intramuscular preadipocytes. The mRNA expression levels of ACVR2B, IL-6R and IGF-IR were significantly down-regulated in subcutaneous preadipocytes after induction, while that of ACVR2B was gradually up-regulated in intramuscular preadipocytes. 【Conclusion】 These results indicated that, unlike the pattern of fat deposition in vivo, intramuscular preadipocytes cultured in vitro showed a higher capability of differentiation than that of subcutaneous preadipocytes. These may be partly related to the lack of inhibition of some potential factors secreted by muscle during intramuscular preadipocyte differentiation." @default.
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- W2382722018 title "Differential mRNA Expression Profiles of Porcine Intramuscular Preadipocytes Compared with Subcutaneous Preadipocytes During Differentiation" @default.
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