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- W2382744514 abstract "OBJECTIVE To analyze the property of recombinant protein Eg.EF-1 from clone Eg.EF-1 gene,and predict its antigen epitopes.METHODS After sequencing the cloned Eg.EF-1 gene,its recombined protein Eg.EF-1 was expressed,then its property and 3D construction was deduced by the tool of Anthe 5.0 and spdbv37sp5.Its amino acid sequence homology was compaired by NCBI/BLAST,and its epitopes was predicted by DNAStar/Protean,BIOSUN and HLA Ligand/Motif software with bioinformatics on line.RESULTS The open reading frame(ORF)of Eg.EF-1 coding a peptide consisted of 230 amino acid and 98% of them coincidenced with the EF-1 from Italian sheep in GeneBank;the recombinant protein Eg.EF-1 was acid with α-helix,the predicted epitopes by three softwares located on five peptides were 20～26(SATEQKG),50～56(SWNERME),176～185(LWGTSKLVPL),199～205(VENDKVG),217～229(ELEDYVQSVDVAS).CONCLUSION Recombinant protein Eg.EF-1 is related protein of the human Eg.EF-1.The epitopes predicted by three bioinformatics tool provided a base for peptide synthesis as hydatid polypeptide vaccine." @default.
- W2382744514 created "2016-06-24" @default.
- W2382744514 creator A5014380157 @default.
- W2382744514 date "2008-01-01" @default.
- W2382744514 modified "2023-09-23" @default.
- W2382744514 title "Property Analysis and Antigen Epitope Prediction of Eg.EF-1 from Human in Ningxia" @default.
- W2382744514 hasPublicationYear "2008" @default.
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