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- W2382767255 abstract "ObjectiveTo screen for the optimal washing buffer in R-phycoerythrin fluorescence immunoassay MethodsChange the ionic intensity and pH value of the washing buffer. Screen the washing buffer by comparing the checkout ratio of positive samples and the pseudo-positive ratio of negative samples.ResultsIncreasing ionic intensity can be helpful to decrease pseudopositive phenomenon in negative samples and to increase sensitivity when concentration is below 120 mmol/L. Pseudopositive ratio can be decreased to zero. when concentration is near to 120 mmol/L and the positive checkout ratio can be up to 93.3%. But much higher concentrations may restrict the sensitivity to be increased. A weak basic washing condition is appropriate.Conclusion120 mmol/L PBS (pH7.5) is suitable for R-phycoerythrin fluorescence immunoassay both in the diagnosis specificity and in the sensitivity. [" @default.
- W2382767255 created "2016-06-24" @default.
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- W2382767255 date "2001-01-01" @default.
- W2382767255 modified "2023-09-23" @default.
- W2382767255 title "Screening for washing condition in solid-phase R-phycoerythrin fluorescence immunoassay" @default.
- W2382767255 hasPublicationYear "2001" @default.
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