Matches in SemOpenAlex for { <https://semopenalex.org/work/W2382783134> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2382783134 abstract "To express ORF3 gene of porcine circovirus 2(PCV-2)in E.coli,analyze the antigenicity of ORF3 protein,according to the amino acid sequence of PCV-2 strain in GenBank,a pair of specific primer was designed to amplify the fragment encoding ORF3 gene.This fragment was cloned into the multiple cloning sites of pET-32a(+)vector.The recombinant plasmid was designated pET32a-ORF3 and was transformed into E.coli Rosetta-blue(DE3)pLacⅠ competent cells.Expression of recombinant protein was induced by addition of final concentration of 1.0 mmol/L IPTG under 37 ℃ condition.The length of PCR product of interest was 315 base pairs.The size of recombinant amino acid was 29 ku.The results of western blotting showed that it could be recognized by anti-His tagged mouse monoclonal antibody and could not be recognized by swine serum infected with PCV-2.The results showed that ORF3 of PCV-2 was expressed successfully in E.coli and the ORF3 protein didn't exhibit reaction genicity.These results were benefit to research for function and characteristics of ORF3 of PCV-2." @default.
- W2382783134 created "2016-06-24" @default.
- W2382783134 creator A5002977087 @default.
- W2382783134 date "2010-01-01" @default.
- W2382783134 modified "2023-09-27" @default.
- W2382783134 title "Cloning and Expression of ORF3 Gene of Porcine Circovirus Type 2 in E.coli" @default.
- W2382783134 hasPublicationYear "2010" @default.
- W2382783134 type Work @default.
- W2382783134 sameAs 2382783134 @default.
- W2382783134 citedByCount "0" @default.
- W2382783134 crossrefType "journal-article" @default.
- W2382783134 hasAuthorship W2382783134A5002977087 @default.
- W2382783134 hasConcept C104317684 @default.
- W2382783134 hasConcept C121050878 @default.
- W2382783134 hasConcept C153911025 @default.
- W2382783134 hasConcept C159047783 @default.
- W2382783134 hasConcept C159654299 @default.
- W2382783134 hasConcept C170344550 @default.
- W2382783134 hasConcept C199360897 @default.
- W2382783134 hasConcept C22744801 @default.
- W2382783134 hasConcept C2522874641 @default.
- W2382783134 hasConcept C2779026604 @default.
- W2382783134 hasConcept C2779231742 @default.
- W2382783134 hasConcept C2781045504 @default.
- W2382783134 hasConcept C33161422 @default.
- W2382783134 hasConcept C40767141 @default.
- W2382783134 hasConcept C41008148 @default.
- W2382783134 hasConcept C54355233 @default.
- W2382783134 hasConcept C79029880 @default.
- W2382783134 hasConcept C86803240 @default.
- W2382783134 hasConceptScore W2382783134C104317684 @default.
- W2382783134 hasConceptScore W2382783134C121050878 @default.
- W2382783134 hasConceptScore W2382783134C153911025 @default.
- W2382783134 hasConceptScore W2382783134C159047783 @default.
- W2382783134 hasConceptScore W2382783134C159654299 @default.
- W2382783134 hasConceptScore W2382783134C170344550 @default.
- W2382783134 hasConceptScore W2382783134C199360897 @default.
- W2382783134 hasConceptScore W2382783134C22744801 @default.
- W2382783134 hasConceptScore W2382783134C2522874641 @default.
- W2382783134 hasConceptScore W2382783134C2779026604 @default.
- W2382783134 hasConceptScore W2382783134C2779231742 @default.
- W2382783134 hasConceptScore W2382783134C2781045504 @default.
- W2382783134 hasConceptScore W2382783134C33161422 @default.
- W2382783134 hasConceptScore W2382783134C40767141 @default.
- W2382783134 hasConceptScore W2382783134C41008148 @default.
- W2382783134 hasConceptScore W2382783134C54355233 @default.
- W2382783134 hasConceptScore W2382783134C79029880 @default.
- W2382783134 hasConceptScore W2382783134C86803240 @default.
- W2382783134 hasLocation W23827831341 @default.
- W2382783134 hasOpenAccess W2382783134 @default.
- W2382783134 hasPrimaryLocation W23827831341 @default.
- W2382783134 hasRelatedWork W2230069488 @default.
- W2382783134 hasRelatedWork W2285615348 @default.
- W2382783134 hasRelatedWork W2365192187 @default.
- W2382783134 hasRelatedWork W2379784772 @default.
- W2382783134 hasRelatedWork W2380927272 @default.
- W2382783134 hasRelatedWork W2380963746 @default.
- W2382783134 hasRelatedWork W2387358509 @default.
- W2382783134 hasRelatedWork W2393864532 @default.
- W2382783134 hasRelatedWork W2402042314 @default.
- W2382783134 hasRelatedWork W2409611724 @default.
- W2382783134 hasRelatedWork W2471092251 @default.
- W2382783134 hasRelatedWork W2553760270 @default.
- W2382783134 hasRelatedWork W2883058614 @default.
- W2382783134 hasRelatedWork W2977927745 @default.
- W2382783134 hasRelatedWork W3004604136 @default.
- W2382783134 hasRelatedWork W3010556497 @default.
- W2382783134 hasRelatedWork W2740420554 @default.
- W2382783134 hasRelatedWork W2868167415 @default.
- W2382783134 hasRelatedWork W2975276270 @default.
- W2382783134 hasRelatedWork W3029778151 @default.
- W2382783134 isParatext "false" @default.
- W2382783134 isRetracted "false" @default.
- W2382783134 magId "2382783134" @default.
- W2382783134 workType "article" @default.