Matches in SemOpenAlex for { <https://semopenalex.org/work/W2382816134> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2382816134 abstract "To get recombinant antigen (Is/et Cell Autoantigen 69)ICA69 which was expressed in Escherichia coli strains (E.coli) by means of the gene engineering technique so that it can be used for early diagnosis of and screening in type Ⅰ diabetes mellitus, the cDNA fragment of human ICA69 was amplified by PCR, and then cloned into pSPORT 1 vector. After DNA sequencing, it was inserted into pGEX-2T between the sites of EcoR Ⅰ and Sma Ⅰ, then recombinant plasmid p2T-ICA69 was constructed and introduced into E.coli. The GST-ICA69 fusion protein was expressed by the induction of IPTG. The recombinant ICA69 proteins were used to detect the antibodies against hICA69 in 100 healthy subjects and type Ⅰ diabetic serum by the use of indirect ELISA. The sequence analysis showed that the amplified fragments contained 1449 bp, encoded 483 amino acids, and had been correctly inserted into pGEX-2T vector. The recombinant proteins expressed in the prokaryotic cells had immunogenicity and could be used to detect antibodies against ICA69 in type Ⅰ diabetic serum. Finally it can be concluded in this paper that the expression products obtained by the method of gene engineering are recombinant ICA69 antigen and may be used to improve the forecast rate and the diagnostic rate of type Ⅰ diabetes in combination with other tests." @default.
- W2382816134 created "2016-06-24" @default.
- W2382816134 creator A5088996718 @default.
- W2382816134 date "2002-01-01" @default.
- W2382816134 modified "2023-09-25" @default.
- W2382816134 title "Preparation of Recombinant Islet Cell Autoantigen 69 kD Fusion Protein" @default.
- W2382816134 hasPublicationYear "2002" @default.
- W2382816134 type Work @default.
- W2382816134 sameAs 2382816134 @default.
- W2382816134 citedByCount "0" @default.
- W2382816134 crossrefType "journal-article" @default.
- W2382816134 hasAuthorship W2382816134A5088996718 @default.
- W2382816134 hasConcept C104317684 @default.
- W2382816134 hasConcept C123894998 @default.
- W2382816134 hasConcept C147483822 @default.
- W2382816134 hasConcept C153911025 @default.
- W2382816134 hasConcept C159654299 @default.
- W2382816134 hasConcept C170344550 @default.
- W2382816134 hasConcept C187882448 @default.
- W2382816134 hasConcept C22744801 @default.
- W2382816134 hasConcept C2780868878 @default.
- W2382816134 hasConcept C33161422 @default.
- W2382816134 hasConcept C40767141 @default.
- W2382816134 hasConcept C54355233 @default.
- W2382816134 hasConcept C547475151 @default.
- W2382816134 hasConcept C55493867 @default.
- W2382816134 hasConcept C86803240 @default.
- W2382816134 hasConceptScore W2382816134C104317684 @default.
- W2382816134 hasConceptScore W2382816134C123894998 @default.
- W2382816134 hasConceptScore W2382816134C147483822 @default.
- W2382816134 hasConceptScore W2382816134C153911025 @default.
- W2382816134 hasConceptScore W2382816134C159654299 @default.
- W2382816134 hasConceptScore W2382816134C170344550 @default.
- W2382816134 hasConceptScore W2382816134C187882448 @default.
- W2382816134 hasConceptScore W2382816134C22744801 @default.
- W2382816134 hasConceptScore W2382816134C2780868878 @default.
- W2382816134 hasConceptScore W2382816134C33161422 @default.
- W2382816134 hasConceptScore W2382816134C40767141 @default.
- W2382816134 hasConceptScore W2382816134C54355233 @default.
- W2382816134 hasConceptScore W2382816134C547475151 @default.
- W2382816134 hasConceptScore W2382816134C55493867 @default.
- W2382816134 hasConceptScore W2382816134C86803240 @default.
- W2382816134 hasLocation W23828161341 @default.
- W2382816134 hasOpenAccess W2382816134 @default.
- W2382816134 hasPrimaryLocation W23828161341 @default.
- W2382816134 hasRelatedWork W2276385301 @default.
- W2382816134 hasRelatedWork W2348991363 @default.
- W2382816134 hasRelatedWork W2352186925 @default.
- W2382816134 hasRelatedWork W2367342778 @default.
- W2382816134 hasRelatedWork W2368129263 @default.
- W2382816134 hasRelatedWork W2371728522 @default.
- W2382816134 hasRelatedWork W2375360629 @default.
- W2382816134 hasRelatedWork W2402744584 @default.
- W2382816134 hasRelatedWork W2407145012 @default.
- W2382816134 hasRelatedWork W2410714667 @default.
- W2382816134 hasRelatedWork W2411044105 @default.
- W2382816134 hasRelatedWork W2415911233 @default.
- W2382816134 hasRelatedWork W2978702265 @default.
- W2382816134 hasRelatedWork W3029110774 @default.
- W2382816134 hasRelatedWork W3031527199 @default.
- W2382816134 hasRelatedWork W3032829938 @default.
- W2382816134 hasRelatedWork W3146107517 @default.
- W2382816134 hasRelatedWork W2800221143 @default.
- W2382816134 hasRelatedWork W2875310867 @default.
- W2382816134 hasRelatedWork W3032166192 @default.
- W2382816134 isParatext "false" @default.
- W2382816134 isRetracted "false" @default.
- W2382816134 magId "2382816134" @default.
- W2382816134 workType "article" @default.