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• W2382816144 abstract "Nasopharyngeal carcinoma (NPC) poses serious health problems in Southern China and yet the molecular mechanism of the carcinogenesis remains unclear. We used modern proteomic technologies to compare the protein expression profiles between the NPC cell lines ( HNE1 and CNE1) and an immortalized nasopharyngeal epithelial cell line NP69 to identify cancer related proteins. Cell lysates were separated by two2 dimensional gel electrophoresis ( 2 DE) and analyzed by PDQuest software. The differentially expressed proteins were identified by matrix2assisted laser desorptionP ionization tandem time2of2flight mass spectrometry (MALDI2TOFP TOF2MS) . We discovered 15 up2regulated proteins and 18 down2regulated proteins in both HNE1 and CNE1 cell lines compared with NP69. These proteins are correlative with various functions , such as cell proliferation , apoptosis , cancer metastasis , metabolism , cytoskeleton and signal transduction. Western blotting analyses were further carried out to verify the differential expression of individual proteins. Several identified proteins in our research might be used as potential molecular markers to understand the molecular mechanism of NPC development and metastasis , and might be used as candidate targets for NPC treatments." @default.
• W2382816144 created "2016-06-24" @default.
• W2382816144 creator A5012072853 @default.
• W2382816144 date "2008-01-01" @default.
• W2382816144 modified "2023-09-26" @default.
• W2382816144 title "Comparative Proteome Analysis of Nasopharyngeal Carcinoma Cell Lines with an Immortalized Nasopharyngeal Epithelial Cell Line NP69" @default.
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• W2382816144 hasPublicationYear "2008" @default.
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