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- W2382821985 abstract "Objective To observe the damage of enteric cholinergic/nitrergic nerve-interstitial cells of Cajal(ICC)network in rats with multiple organ dysfunction syndrome(MODS).Methods Forty Wistar rats were randomly divided into control group(n=20)and MODS model group(n=20).The cholinergic/nitrergic nerve-ICC network in the intestine was observed using c-Kit and VAChT/nNOS im- munohistochemical double-staining with whole-mount preparation technique and confocal laser scanning microscopy.Results In control group,the number of c-Kit-positive ICCs,VAChT/nNOS-positive fibers in one optical section was(18.0±2.2),(28.0±1.3)and(16.0±0.3),respectively,and the inten- sity of immunoreactivity was(30.0±3.4),(208.0±10.4)and(222.0±4.4)U,respectively.In the MODS group,the number of c-Kit-positive ICCs,VAChT/nNOS-positive fibers in one optical section was (6.0±0.5),(13.0±1.1)and(7.0±0.5),respectively,and the intensity of immunoreactivity was (8.0±1.2),(98.0±9.1)and(96.0±7.9)U,respectively.Compared with those in control group, the distributions and densities of cholinergic/nitrergic nerves and ICC in the intestine of model group were significantly decreased(P0.01),and the enteric nerve-ICC network was discontinued.Conclusion The enteric nerve-ICC network was disrupted in MODS,which may be associated with the disorder of gas- trointestinal motility." @default.
- W2382821985 created "2016-06-24" @default.
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- W2382821985 date "2007-01-01" @default.
- W2382821985 modified "2023-09-28" @default.
- W2382821985 title "Changes of enteric nerve-interstitial cells of Cajal network in rats with multiple organ dysfunction syndrome" @default.
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