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- W2382824074 abstract "Objective:To detect the expression of PCNP in the splenocytes of cattle collagen Ⅱ induced RA mouse models and the lymphocytes from peripheral blood of patients with RA and discuss the role PCNP plays in the procession of RA.Methods:DBA-1/j mice were immunized by collagen Ⅱ to establish CIA mouse models.Patients with RA were divided into resting group and active group.Semiquantitative RT-PCR and fluorescence quantitative RT-PCR were employed to detect the expression difference of PCNP genes in the splenocytes of CIA mice compared with that of normal mice and in the lymphocytes of peripheral blood from patients with RA compared with that from the health controls.The expression difference of PCNP proteins in the splenocytes of CIA mice compared with the normal mice and in the lymphocytes of peripheral blood from patients with RA compared with the health controls was detected by Western blot.Results:The expression of PCNP genes and proteins was increase in the splenocytes of CIA mice and in the lymphocytes of peripheral blood from patients with RA compared with relative control group.Furthermore,among patients with RA,the expression of PCNP genes and proteins in active group was more than that in resting group.All the differences were statistical significance.Conclusion:At gene and protein levels,the expression of PCNP in the splenocytes of CIA mice and in the lymphocytes of peripheral blood from patients with RA was significantly more than that in the health controls.The expression amount was closely related to severity of RA." @default.
- W2382824074 created "2016-06-24" @default.
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- W2382824074 date "2013-01-01" @default.
- W2382824074 modified "2023-09-25" @default.
- W2382824074 title "The expression and significance of PCNP in rheumatoid arthritis" @default.
- W2382824074 hasPublicationYear "2013" @default.
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