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- W2382837402 abstract "Objective To develop a rapid method to detect Staphylococcus aureus(S.aureus) from complex bacterial samples by capillary electrophoresis(CE) in order to meet the need of rapid diagnosis of infectious disease.Methods By background buffer added with S.aureus specific monoclonal antibody-coated latex,electromigration trait of S.aureus was changed and separation of target bacteria was achieved by CE.Results S.aureus could be selectively isolated from complex bacterial mixture containing Escherichia coli,Pseudomonas aeruginosa and Klebsiella pneumoniae in 20 min.Stool sample from a healthy volunteer artificially spiked with S.aureus was subjected to analysis.Bacteria eluted out at different time were collected and then cultured by traditional methods.The results showed that the developed method was reliable.Conclusions CE coupled with antibody-coated latex method is a rapid S.aureus detection strategy and valuable for further development and application." @default.
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- W2382837402 date "2009-01-01" @default.
- W2382837402 modified "2023-09-24" @default.
- W2382837402 title "A rapid method for the detection of Staphylococcus aureus by capillary electrophoresis" @default.
- W2382837402 hasPublicationYear "2009" @default.
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