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- W2382840082 abstract "A protocol using Ion Exchange Chromatography (IEC), Hydrophobic Interaction Chromatography (HIC) and Affinity Chromatography for purifing recombinant human Growth Hormone (rhGH) expressed in insect cells was reported. IEC and HIC was used as the initial step for concentration and partial purification. The rhGH was extracted from insect cell culture fluid by batch absorption to the series-connected columns (CM-52 and Phenyl Sepharose CL-4B) in sequence. This procedure rendered a concentrated solution. The following step was Affinity Chromatography, which shows high efficiency of purification and recovery. The purified rhGH was determined to have identical sequence with native one by Tryptic peptide map; and also have identical immuitive activity by Western blotting; molecular weight of 22,000 daltons by SDS-PAGE; isoelectric point of pH 4.9 by isoelectric focus; Phe as N-terminal amino acid by DNS-CL terminal assaying method. All the properties conform with those of native hGH." @default.
- W2382840082 created "2016-06-24" @default.
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- W2382840082 date "2002-01-01" @default.
- W2382840082 modified "2023-09-25" @default.
- W2382840082 title "PURIFICATION OF RECOMBINANT HUMAN GROWTH HORMONE EXPRESSED IN INSECT CELLS" @default.
- W2382840082 hasPublicationYear "2002" @default.
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