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- W2382848041 abstract "Aim: To detect the airway inflammation and Mucin5AC in rats with chronic obstructive pulmonary disease (COPD).Methods:Twenty Wistar rats were divided randomly into 2 groups: normal group (group N, n=10) and the model of COPD group (group C, n=10). The COPD model was established through passive smoking and intratracheal instillation of LPS. BALF was collected to count inflammatory cells after 6 weeks. Immunohistochemistry was processed to observe the goblet cell and the expression of Mucin5AC in airway tissue of the rats.Results: BALF analysis presented that PMN count was higher in group C ((251.10±42.76)×10~6 L~ -1 ) than that in group N (( 17.58 ±3.72)×10~6 L~ -1 ) and there was significant difference; airway pathological score was higher in group C (69.30±4.99) than that in group N ( 18.56 ±2.46) and there was significant difference; alveolar diameter was longer in group C ((92.58±4.32)μm) than that in group N ((47.22±12.95)μm) and there was significant difference. DAB staining presented that goblet cell hyperplasia index in group C ((51.20±9.23)/mm~2) was higher than that in group N ((5.12±3.07)/mm~2) and there was significant difference; Mucin5AC was overexpressed in group C ((34.50± 6.52 )%) and almost absent in group N (( 3.12 ±2.60)%) and there was significant difference. The PMN count was correlated with the airway pathological score.Conclusion: The results suggest that many inflammatory cells involve in the development and progress of COPD." @default.
- W2382848041 created "2016-06-24" @default.
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- W2382848041 date "2007-01-01" @default.
- W2382848041 modified "2023-09-27" @default.
- W2382848041 title "Detection of airway inflammation and Mucin5AC in rats with chronic obstructive pulmonary disease" @default.
- W2382848041 hasPublicationYear "2007" @default.
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