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- W2382849827 abstract "The glucosyltransferase gene promoter fragment B,which cloned from tobacco by the lab early and induced by both methyl jasmonate and salicylic acid,was fused to the 5′-upstream of GUS(β-glucuronidase) coding region in binary vector,designated pBA,BB,BC and BD-GUS.The pB-GUS was introduced into W38 tobacco plant by Agrobacterium tumefaciens.The positive ratio of the transgenic tobacco plants with different promoter fragments was 50%～71%.Quantification analysis of GUS activity induced showed that the basic expression of the promoter fragment BA was very low,and the expression was strongly induced by both MeJA and SA.The basic expression of the promoter fragments BB and BC was relatively high,and the different transgenic plants with pBB and BC-GUS showed different response to MeJA and SA.The basic expression of the fragment BD was higher than that of BA,but lower than that of BB and BC,and the GUS activity in transgenic plants with pBD-GUS all were induced by SA.These results showed that in the region of-756～-703(BA) might be there was an element induced by both MeJA and SA,and-643～-606 with an element induced by SA.The identification of these elements in this promoter would help to know the principle of inducing mechanism by MeJA and SA." @default.
- W2382849827 created "2016-06-24" @default.
- W2382849827 creator A5041453553 @default.
- W2382849827 date "2009-01-01" @default.
- W2382849827 modified "2023-09-26" @default.
- W2382849827 title "Cloning and Induced Expression of the Promoter Fragment B of a Glucosyltransferase Gene in Tobacco" @default.
- W2382849827 hasPublicationYear "2009" @default.
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