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- W2382849907 abstract "To explore a rapid,effective method to separate rat ovary theca cells by density gradient centrifu-gation.The ovaries of 23~25-days-old were collected.The theca cells were separated by Percoll density gra-dient centrifugation procedure.The purity of theca cell was examined by 3β-hydroxysteroid dehydrogenase(3β-HSD) staining.The separated cells were cultured for 48 h under serum-free cell culture condition with 0.1 U/mL or 1.0 U/mL FSH or LH,then the concentration of androstenediol and estradiol of the culture medium were measured by enzyme-linked immunosorbent assay.In the separated cells,the ratio of 3β-HSD positive cells to the total cells was more than 90%.The level of androstenediol in LH cultured media was significantly higher than that of the FSH cultured and control media(P0.05);In LH cultured media,the level of androstenediol in 1.0 U/mL LH was significantly higher than that of in 0.1 U/mL LH.The level of androstenediol was no difference between FSH cultured media and control media(P0.05).3β-HSD staining could be applied as a rapid and effective method to examine the purity of the separated theca cells.The procedure described by this study can separate rat ovary theca cells which is reacted with LH stimulation and barely mixed with contaminated graunlosa cells." @default.
- W2382849907 created "2016-06-24" @default.
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- W2382849907 date "2011-01-01" @default.
- W2382849907 modified "2023-09-24" @default.
- W2382849907 title "Separation of Rat Ovary Theca Cells by Density Gradient Centrifugation" @default.
- W2382849907 hasPublicationYear "2011" @default.
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