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- W2382882052 abstract "OBJECTIVE:To establish a simple,reasonable and reliable method for the content determination of polydatin,emodin and physcion in Polygonum cuspidatum. METHODS:HPLC method was adopted The determination was performed on Agilent TC-C18(250 mm×4.6 mm,5 μm)column with mobile phase consisted of 0.1% formic acid-acetonitrile(gradient elution)at flow rate of 1 ml/min. The column temperature was 25℃ and the detection wavelengths were set at 254 nm(emodin,physcion)and 319 nm(polydatin). Maflab 7.1 programming was used for chromatogram fusion;using emodin as internal standard,the relative correction factors of polydatin and physcion were established to calculate the contents of polydatin and physcion. The contents of 3 components from different producing areas calculated by correction factor method were compared with the measured values of external standard method. RESULTS:The linear ranges of polydatin,emodin and physcion were 0.29-1.76 μg(r=0.999 0),0.75-4.51 μg(r=0.999 8)and 0.05-0.31 μg(r=0.999 6). RSDs of precision,reproducibility and stability tests were all lower than 3%. Average recovery rates were 98.50%(RSD=1.81%,n=6),101.68%(RSD=2.03%,n=6)and 99.22%(RSD=2.44%,n=6). The contents of 3 components in P. cuspidatum by above 2 methods had no significant difference. CONCLUSIONS:The method is simple,reasonable and reliable for quality control of P. cuspidatum." @default.
- W2382882052 created "2016-06-24" @default.
- W2382882052 creator A5045094644 @default.
- W2382882052 date "2013-01-01" @default.
- W2382882052 modified "2023-09-23" @default.
- W2382882052 title "Content Determination of 3 Components of Polygonum cuspidatum by HPLC Dual Wavelength Baseline Fusion Combined with Correction Factor" @default.
- W2382882052 hasPublicationYear "2013" @default.
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