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- W2382948181 abstract "CjAPL1 gene cloned from Camellia japonica is the AP1 homologous gene belonged to MADS-box gene family.And AP1 could have important regulatory function to floral organs development.The fragment length of 292bp in CjAPL1 gene was selected and used to construct RNA interference intermediate vector with inverted repeat sequence pUCC-CjAPL1 by double enzyme digestion.The pUCC-CjAPL1 vector and PCAMBIA1300 expression vector were respectively digested by Pst I.Then small fragment with inverted repeat sequence and larger fragment in pCAMBIA1300 achieved were connected to construct pCAM-RNAiCjAPL1 expression vector finally.The vector was transformed to Agrobacterium tumefaciens GV3101 and then guided into Arabidopsis thaliana wild type by inflorescence impregnation.The positive plants with hygromycin resistance were dealt with PCR amplification and phenotypic observation statistics.The result showed that pCAMRNAi-CjAPL1 expression vector was completely constructed and successfully guided into genome of Arabidopsis thaliana.The positive plants showed floral organs change of 1 or 2 stamens deletion.This indicated that CjAPL1 gene could regulate and control the floral organs(such as stamen,and so on) development." @default.
- W2382948181 created "2016-06-24" @default.
- W2382948181 creator A5008494399 @default.
- W2382948181 date "2013-01-01" @default.
- W2382948181 modified "2023-09-23" @default.
- W2382948181 title "RNA Interference Expression Vector Construction of CjAPL1 Gene from Camellia japonica and Genetic Transformation" @default.
- W2382948181 hasPublicationYear "2013" @default.
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