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- W2382972482 abstract "Objective To understand the characteristics of protein fractions of Schistosoma japonicum(Sj) reaction with sera of Microtus fortis(Mf) for identification the provision of protective antigen. Methods The proteins of Sj different stages,SLWP,SHWP,SAWP and SSP,were prepared and separated by SDS-PAGE and stained by PAS for saccharides or by Coomassie brilliant blue.Proteins fractions reacted with sera of Mf with Western blot.The molecular weight range of positive bands was measured,and then comparatively analyzed their characteristic according to the standard protein. Results Significant difference of worm protein fractions among four different developmental stages of Sj recognized by Mf sera was seen in volume and molecular weight.Of them,we observed that only 110 kDa fractions in SSP,the 110 kDa and 45～50 kDa components in SLWP,150 kDa,110 kDa,22 kDa,and 14 kDa in SHWP,and 110 kDa,80/82 kDa,90/92 kDa,45 kDa,22 kDa,and 14 kDa in SAWP were recognized.In reaction strength,positive bands in SLWP and SHWP were the most significant.Comparison of these components with the corresponding protein electrophoresis bands stained with the glycoprotein showed that protein 110 kDa in 4 different developmental stages worm and 45 kDa,22 kDa and 14 kDa in SAWP belong to the glycoprotein. Conclusions There are a variety of natural antibodies against Sj protein components in serum of Mf.The main components of them show in the lung-stage and liver-stage schistosomulum and display glycoprotein characteristics." @default.
- W2382972482 created "2016-06-24" @default.
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- W2382972482 date "2009-01-01" @default.
- W2382972482 modified "2023-09-25" @default.
- W2382972482 title "Analysis on Characteristics of Protein Component of Schistosoma Japonicum Different Developmental Stages Recognition by Serum of Microtus Fortis" @default.
- W2382972482 hasPublicationYear "2009" @default.
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