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- W2382991441 abstract "[Objective]To construct and express human maff gene expression vector in human hepatoma cell line HepG2 cells.[Methods]human maffgene was amplificated by PCR and beenconnected with cloning plasmids then transfectedinto E.coli and amplified,identify with restriction enzyme digestion,and then connect human maff gene with expression vector and transfected into human hepatomacell line HepG2 cells express and identify with westernblot.[Results]Identify with restriction enzyme digestion and westernblot,humanmaff gene expression vector can express Maf F protein in eukaryotic cells.[Conclusions]this research construct maff gene eukaryoti cexpression vector and provide the experimental basis for the prevention with traditional Chinese medicine to the tumor." @default.
- W2382991441 created "2016-06-24" @default.
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- W2382991441 date "2010-01-01" @default.
- W2382991441 modified "2023-10-03" @default.
- W2382991441 title "Studyon the Construction of Human maff Gene Expression Vector" @default.
- W2382991441 hasPublicationYear "2010" @default.
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