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- W2383048795 abstract "Objective:To explore the practicability of in-situ cryopreservation for mesenchymal stem cells(MSCs) and its effect on cell viability, the later migrating passage and capability of osteogenic differentiation.Methods:β-TCP 3D Scaffolds were produced.MSCs amplification in 3D environment was investigated in comparison with 2D.The harvest and survival rate, as well as the migration rate, were tested by CCK-8 reagent staining for cell number calculation.ALP activity and specific ALP activity were examined for both groups of the scaffold-cells constructs, which had experienced the in-situ cryopreservation or not.Results:The 3D propagation speed was about 7 times than that of the 2D.The harvest and survival rate had risen from 55.9% to 81.3% when the 3D in-situ cryopreservation was applied instead of the traditional 2D trypsinization and cryopreservation.The migration rate of the cryopreservation group was not less than the non-cryopreservation group.Interestingly, the specific ALP activity of the cryopreservation passage group was even higher than the non-cryopreservation group.Conclusions:Employing β-TCP 3D Scaffolds for MSC propagation, passage and in-situ cryopreservation was applicable and promising." @default.
- W2383048795 created "2016-06-24" @default.
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- W2383048795 date "2009-01-01" @default.
- W2383048795 modified "2023-09-25" @default.
- W2383048795 title "β-TCP 3D Scaffolds for Mesenchymal Stem Cells in-situ Cryopreservation" @default.
- W2383048795 hasPublicationYear "2009" @default.
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