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- W2383067609 abstract "Objective To evaluate the effects of resveratrol(Res) on expression and activity of matrix-metalloproteinase(MMP)-2 in glioma U87 cells.Methods Activity and expression of MMP-2 in U87 cells were detected by gelatin zymography assay and Western blotting,respectively;immunocytochemistry was performed to determine the effect of Res on transcriptional activity of nuclear factor(NF-κB).Results As gelatin zymography assay showed,the activity of MMP-2 was decreased significantly in U87 cells treated by 40 μM Res for 4 h,24 h and 48 h(P0.05).Western blotting confirmed Res-induced inhibition of MMP-2 protein expression(P0.05).Results from immunocytochemistry showed the translocation of p65 from cytoplasm to nucleus was blocked by 40 μM Res treatment(P0.05).In addition,SN50,a NF-κB inhibitor,reduced both the activity and protein expression of MMP-2 in U87 cells(P0.05).Conclusion Activity and expression of MMP-2 can be inhibited by 40 μM Res in U87 cells,which may be attributed to Res-induced suppression of NF-κB transcriptional activity." @default.
- W2383067609 created "2016-06-24" @default.
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- W2383067609 date "2013-01-01" @default.
- W2383067609 modified "2023-09-23" @default.
- W2383067609 title "Effects of resveratrol on activity and expression of MMP-2 in glioma U87 cells" @default.
- W2383067609 hasPublicationYear "2013" @default.
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