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- W2383075900 abstract "A method of magnetic-bead hybridization enrichment was used to isolate the microsatellite loci of Portunus trituberculatus.Genomic DNA was digested with restriction enzyme Sau3A1,and DNA fragments in range of 500— 1000bp was agarose-gel purified and ligated to the Sau3A1 adaptor.The fragments were hybridized with biotin-labelled(GT)15 probe and captured by Streptavidin-coated magnetic beads.The target fragments were eluted,PCR amplified,inserted to PMD18-T vector,and transformed into DH5α component cell.Clones in enriched genomic DNA bank were screened through PCR method.Of the 56 sequenced clones,49 sequences contained microsatellite having at least 5 times repeats numbers.Among 47 non-redundant microsatellite-contained sequences,about 87.5% of all the sequences,the largest repeat number of perfect type microsatellite was 47,of which 40 had enough flanking region(80—100bp) for designing primer pairs,and will be tested for polymorphism in the future." @default.
- W2383075900 created "2016-06-24" @default.
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- W2383075900 date "2009-01-01" @default.
- W2383075900 modified "2023-09-23" @default.
- W2383075900 title "MICROSATELLITE LOCI OF PORTUNUS TRITUBERCULATUS:ISOLATION AND CHARACTERIZATION" @default.
- W2383075900 hasPublicationYear "2009" @default.
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