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- W2383080924 abstract "Strain LC47,a bacteriocin-like substance producing strain,was screened from yellow-liquor by scrip method and cylinder-dish method,and was identified as Lactobacillus buchneri.The supernatant of LC47 culture exhibited strong inhibitory activity against the gram-positive bacteria,such as Staphylococcus aureus and Bacillus subtilis,the gram-negative bacteria,such as Escherichia coli and Pseudomonas pyocyanea,and also Saccharomyces cerevisiae and some epiphyte,after the effects of organic acids and hydrogen peroxide were eliminated.The relative potency of supernatant increased by 216% and had stable inheritance,after LC47 was treated with repeated multiple mutation with ultraviolet radiation(UV) and diethyl sulphate(DES).The study on fermentation conditions of such bacteriocin-like substance producing bacteria showed that the maximum inhibitory activity could be reached under such conditions: 2% maltose as carbon source,1% peptone added by 0.5% yeast extract as nitrogen source,culture temperature 37℃,initial pH 6.0-6.5,1% inoculum,anaerobic cultivation 48 h with K+?Mg2+?NH4+ and Mn2+ exist." @default.
- W2383080924 created "2016-06-24" @default.
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- W2383080924 date "2006-01-01" @default.
- W2383080924 modified "2023-09-28" @default.
- W2383080924 title "Primary study on screening of bacteriocin-like substance producing bacterium LC47 and its fermentation substance" @default.
- W2383080924 hasPublicationYear "2006" @default.
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