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- W2383143362 abstract "Objective To investigate the major genotypes and prevalence of metallo-β-lactamases(MBLs)in Enterobacteriaceae from a hospital in2007-2011.Methods All strains of Enterobacteriaceae were isolated from clinical specimens by routine method,antimicrobial susceptibility testing was performed by disc diffusion method(KirbyBauer)and agar dilution method,MBLs was screened by both modified Hodge test and Etest,MBLs genes were amplified by polymerase chain reaction(PCR),the transference of IMP-8-encoding plasmid was performed by conjugation test,the homology of MBLs-producing strains was detected by pulsed-field gel electrophoresis(PFGE).Results Of 3 297 Enterobacteriaceae isolates,41 were resistant to carbapenems,and phenotype confirmation test showed 14 strains produced carbapenemase,3 Klebsiella pneumoniae isolates carrying blaIMP-8belonged to the same clone,which can be transferred to others by plasmid.Conclusion Three blaIMP-8-carrying Klebsiella pneumoniae isolates were found in this hospital,and has a high degree of homology." @default.
- W2383143362 created "2016-06-24" @default.
- W2383143362 creator A5021654606 @default.
- W2383143362 date "2014-01-01" @default.
- W2383143362 modified "2023-09-28" @default.
- W2383143362 title "Detection of metallo-β-lactamases in clinical isolates of Enterobacteriaceae" @default.
- W2383143362 hasPublicationYear "2014" @default.
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