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- W2383156793 abstract "Objective:To Cloning IGF-1gene which contained not only mature but also signal peptide coded sequence as fundament for study the trans IGF-1gene.Methods:Amˉplified mature and signal peptide coded sequence of IGF-1gene respectively.Connected these two DNA fragments together by PCR and inserted the newly formed360bp DNA fragment into plasmid pGEM-Teasy.Results:The recombinant plasmid contained an exogenous DNA fragˉment of360bp encoding IGF-1signal and mature peptide.Conclusion:The expression of IGF-1gene and a more simple and economic competitive PCR assay for already cloned gene is put forward." @default.
- W2383156793 created "2016-06-24" @default.
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- W2383156793 date "2003-01-01" @default.
- W2383156793 modified "2023-09-23" @default.
- W2383156793 title "Cloning of IGF1 gene containing signal peptide coded sequence" @default.
- W2383156793 hasPublicationYear "2003" @default.
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