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- W2383165290 abstract "The extract and properties of linoleate isomerase in Lactobacillus bulgaricus was studied in this paper.Ultrasonic wave was used to disrupt the cells of Lactobacillus bulgaricus so as to extract linoleate isomerase from the cells,and the influence of the disruption condition to the release of linoleate isomerase was studied.After single-factor experiment and orthogonal experiment,the optimum disruption condition for Lactobacillus bulgaricus was:intermittent ultrasonic treatment 80 times(time for each treatment was 3 s,with a 4 s interval),volume of the treated cell suspension 60 mL,concentration of cell suspension 15 g/50 mL,pH value of the extraction buffer 4.8,concentration of NaCl 0.3 mol/L.Crude enzyme extract from Lactobacillus bulgaricus was purified by ammonium sulfate salting-out,ultrafiltration and gel fractionation chromatography.Purification of about 23.00-fold was achieved.After those steps,there was only one distinct protein band by SDS-PAGE,which showed that the relative molecular weight of linoleate isomerase was about 3 3000 Dal.Some of the enzymatic properties was also studied.Results showed that,linoleate isomerases could specifically transform linolenic acid to conjugated linoleic acid isomers with physiological activities,and the ratio of those isomers amounted to 93.626 %." @default.
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- W2383165290 date "2006-01-01" @default.
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- W2383165290 title "STUDY ON LINOLEATE ISOMERASE OF LACTOBACILLUS BULGARICUS" @default.
- W2383165290 hasPublicationYear "2006" @default.
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