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- W2383165618 abstract "Objective To explore the antibiotics resistance,integrons expression and antibiotics-resistant gene of Aclnetobacter baumannii isolates.Methods 100 strains of Aclnetobacter baumannii were isolated from clinical specimens.VITEK-64 system was used to identify the bacteria and test their susceptibility to 14 antibiotics.Class 1,2 and 3 integron integrase genes(intI 1,2 and 3) were detected by PCR.Inserted gene cassettes of variable regions in intI 1 positive isolates were detected with PCR combined with sequence analysis.Results Except amikacin and cefoperazone/sulbactam,the resistance rate of Aclnetobacter baumannii to other 12 antibiotics was higher than 60.0%,and multiple antibiotics-resistance rate was 88.0%.Integron integrase genes were observed in 64 of 100 strains of Aclnetobacter baumannii;all were intI 1.In integron positive strains,the resistance rate to common antibiotics was much higher than that in integron negative strains.In intI 1 positive strains,variable regions of integron were amplified in 54 of 64;three inserted gene cassettes were observed including aac(6′)-Ib-cr-arr-3-dfrA27(14 strains),aacA4-catB8-aadA1(24 strains),and aacC1-orfA-orfB-aadA1(16 strains).Conclusion Class1 integron expression is associated with multiple antibiotics resistance of Aclnetobacter baumannii in clinical isolates.This class 1 integron mainly carries gene cassettes of resistance to aminoglycosides antibiotics early used,trimethoprim and chloromycetin." @default.
- W2383165618 created "2016-06-24" @default.
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- W2383165618 date "2013-01-01" @default.
- W2383165618 modified "2023-09-26" @default.
- W2383165618 title "Association of integrons and inserted gene with antibiotics resistance of 100 strains of Aclnetobacter baumannii isolates" @default.
- W2383165618 hasPublicationYear "2013" @default.
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