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- W2383206719 abstract "Objective:To study the effects of serum containing Longzhong Sunshang San (SLSS) on proliferation and osteogenic differentiation of bone marrow mysenchymal stem cells (BMSCs) in rats. Methods:BMSCs were separated from bone marrow collected from three-month-old Wistar rats and then co-cultured with 2%,5% and 10% SLSS. Morphological changes were observed with inverted microscope. MTT assay was performed to measure the proliferation of BMSCs. CFU -FALP staining was used to measure the number of CFU-F stained positive for ALP. Von Kossa staining and immunohistochemistry (IHC) techniques were separately used to measure the number of calcification tubercle and the expression of type I collogen. ALP activity and the calcium deposition level were measured at different periods of time. Results:SLSS enhanced the proliferation and osteogenic differentiation of BMSCs in a dose-dependent manner,with strongest effect at 10%. Conclusion:Longzhong Sunshang San could stimulate the proliferation and osteogenic differentiation of BMSCs,which might be one of the mechanisms of Longzhong Sunshang San in promoting the concrescence of bone fracture,preventing and treating osteoporosis." @default.
- W2383206719 created "2016-06-24" @default.
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- W2383206719 date "2010-01-01" @default.
- W2383206719 modified "2023-09-26" @default.
- W2383206719 title "Effects of Serum Containing Longzhong Sunshang San on the Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Rats" @default.
- W2383206719 hasPublicationYear "2010" @default.
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