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- W2383250604 abstract "mtDNA sequence alterations are challenging to generate but desirable for basic studies and potential correction of mtDNA diseases. Here, we report a new method for transferring isolated mitochondria into somatic mammalian cells using a photothermal nanoblade, which bypasses endocytosis and cell fusion. The nanoblade rescued the pyrimidine auxotroph phenotype and respiration of ρ0 cells that lack mtDNA. Three stable isogenic nanoblade-rescued clones grown in uridine-free medium showed distinct bioenergetics profiles. Rescue lines 1 and 3 reestablished nucleus-encoded anapleurotic and catapleurotic enzyme gene expression patterns and had metabolite profiles similar to the parent cells from which the ρ0 recipient cells were derived. By contrast, rescue line 2 retained a ρ0 cell metabolic phenotype despite growth in uridine-free selection. The known influence of metabolite levels on cellular processes, including epigenome modifications and gene expression, suggests metabolite profiling can help assess the quality and function of mtDNA-modified cells." @default.
- W2383250604 created "2016-06-24" @default.
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- W2383250604 date "2016-05-01" @default.
- W2383250604 modified "2023-10-15" @default.
- W2383250604 title "Mitochondrial Transfer by Photothermal Nanoblade Restores Metabolite Profile in Mammalian Cells" @default.
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- W2383250604 doi "https://doi.org/10.1016/j.cmet.2016.04.007" @default.
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