Matches in SemOpenAlex for { <https://semopenalex.org/work/W2383268550> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2383268550 abstract "Objective:To transfer EGFP gene into porcine MSCs by adopting IRE-S2、EGFP vector so as to observe the expression of enhance green fluorescent protein(EGFP) and their growth characters after gene transfer,to provide the evidence for MSCs existing and fates in the future research.Methods:Chinese Laboratory Miniature pigs aged 1 months were used as donors of bone marrow stem cells.We transfered EGFP gene into porcine MSCs.The expression of EGFP was observed and the ratio of transfection was calculated.The expression ratio of EGFP gene and the proliferation ability was detected using FACS after G418 selecting.Results:EGFP expression ratio was 36.9%.EGFP expression was up to 75.6% after G418 selecting.Compared with untransfected control MSCs,the number of cells increased in G0/G1phase(P0.01)and decreased in,S+G2/M phase(P0.01).Conclusion:Steady expression of EGFP is in MSCs by the gene transfer technique and G418 selecting.The proliferate activity is inhibited after gene transfer.The number of cells increase in G0/G1 phase and decrease in S+G2/M phase." @default.
- W2383268550 created "2016-06-24" @default.
- W2383268550 creator A5085204662 @default.
- W2383268550 date "2009-01-01" @default.
- W2383268550 modified "2023-09-24" @default.
- W2383268550 title "Observation of Biological Characteristics of Porcine Mesenchymal Stem Cells after Enhance Green Fluorescent Protein Gene Transfer" @default.
- W2383268550 hasPublicationYear "2009" @default.
- W2383268550 type Work @default.
- W2383268550 sameAs 2383268550 @default.
- W2383268550 citedByCount "0" @default.
- W2383268550 crossrefType "journal-article" @default.
- W2383268550 hasAuthorship W2383268550A5085204662 @default.
- W2383268550 hasConcept C104317684 @default.
- W2383268550 hasConcept C111599444 @default.
- W2383268550 hasConcept C142613039 @default.
- W2383268550 hasConcept C150194340 @default.
- W2383268550 hasConcept C153911025 @default.
- W2383268550 hasConcept C198826908 @default.
- W2383268550 hasConcept C28328180 @default.
- W2383268550 hasConcept C3018928802 @default.
- W2383268550 hasConcept C54009773 @default.
- W2383268550 hasConcept C54355233 @default.
- W2383268550 hasConcept C86803240 @default.
- W2383268550 hasConcept C95444343 @default.
- W2383268550 hasConceptScore W2383268550C104317684 @default.
- W2383268550 hasConceptScore W2383268550C111599444 @default.
- W2383268550 hasConceptScore W2383268550C142613039 @default.
- W2383268550 hasConceptScore W2383268550C150194340 @default.
- W2383268550 hasConceptScore W2383268550C153911025 @default.
- W2383268550 hasConceptScore W2383268550C198826908 @default.
- W2383268550 hasConceptScore W2383268550C28328180 @default.
- W2383268550 hasConceptScore W2383268550C3018928802 @default.
- W2383268550 hasConceptScore W2383268550C54009773 @default.
- W2383268550 hasConceptScore W2383268550C54355233 @default.
- W2383268550 hasConceptScore W2383268550C86803240 @default.
- W2383268550 hasConceptScore W2383268550C95444343 @default.
- W2383268550 hasLocation W23832685501 @default.
- W2383268550 hasOpenAccess W2383268550 @default.
- W2383268550 hasPrimaryLocation W23832685501 @default.
- W2383268550 hasRelatedWork W1521734828 @default.
- W2383268550 hasRelatedWork W155573918 @default.
- W2383268550 hasRelatedWork W2031892163 @default.
- W2383268550 hasRelatedWork W2042034387 @default.
- W2383268550 hasRelatedWork W2348338506 @default.
- W2383268550 hasRelatedWork W2357883836 @default.
- W2383268550 hasRelatedWork W2360152621 @default.
- W2383268550 hasRelatedWork W2368359566 @default.
- W2383268550 hasRelatedWork W2371560450 @default.
- W2383268550 hasRelatedWork W2372381179 @default.
- W2383268550 hasRelatedWork W2373910537 @default.
- W2383268550 hasRelatedWork W2375411384 @default.
- W2383268550 hasRelatedWork W2375698564 @default.
- W2383268550 hasRelatedWork W2378391410 @default.
- W2383268550 hasRelatedWork W2386580646 @default.
- W2383268550 hasRelatedWork W2389763958 @default.
- W2383268550 hasRelatedWork W2391168343 @default.
- W2383268550 hasRelatedWork W2393143859 @default.
- W2383268550 hasRelatedWork W2398014224 @default.
- W2383268550 hasRelatedWork W3032084008 @default.
- W2383268550 isParatext "false" @default.
- W2383268550 isRetracted "false" @default.
- W2383268550 magId "2383268550" @default.
- W2383268550 workType "article" @default.