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- W2383292767 abstract "The accumulation of pullulanase in rice grains was down regulated by RNA interference(RNAi). An RNAi plasmid,which contained an inverted repeat sequences of a pullulanase gene fragment spliced by an intron fragment and driven by an endosperm-specific promoter, was generated. Agrobacterium-mediated rice (Oryza sativa sp. japonica) transformation was conducted and 28 transgenic lines were confirmed containing the designed RNAi construct by PCR and Southern blot. Western analysis of the protein from developing endosperms with antibody against rice pullulanase showed that the accumulation of pullulanase was affected. Rice lines with varying amounts of pullulanase accumulation were obtained. This observation was confirmed by in-gel activity assays of pullulanase. Transgenic line 012 contained approximately 10% pullulanase protein comparing to wildtype, suggesting the RNAi construct from this study can be used to silence pullulanase activity in rice effectively." @default.
- W2383292767 created "2016-06-24" @default.
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- W2383292767 date "2007-01-01" @default.
- W2383292767 modified "2023-09-23" @default.
- W2383292767 title "Down-Regulation of Rice Pullulanase Gene Expression by RNA Interference" @default.
- W2383292767 hasPublicationYear "2007" @default.
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