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- W2383359120 abstract "To investigate mitomycin C(MMC)-induced apoptosis of ASTC-a-1,the cells were treated with MMC at different concentration and their activity was assessed by cell counting kit(CCK-8).Based on results of CCK-8,cells were treated with 10 μg/mL of MMC.Hochest 33258 has been used to verifiy the MMC-induced cell apoptosis and the results indicated that MMC induced cell and nuclear shrinked.For advanced investigation of the apoptosis pathway induced by MMC,human lung adenocarcinoma cells(ASTC-a-1) was transfected with SCAT3 plasmid,a fluorescence resonance energy transfer(FRET) plasmid.Photobleaching and emission spectrum techniques have been used to monitor the activation of Caspase-3 in living cells.ASTC-a-1 was transfected with plasmid Bax and Ds-Red to monitor the translocation of Bax into mitochondria during apoptosis induced by MMC.Our results demonstrate that both Caspase-3 activation and Bax are involved in the MMC-induced apoptosis." @default.
- W2383359120 created "2016-06-24" @default.
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- W2383359120 date "2008-01-01" @default.
- W2383359120 modified "2023-09-23" @default.
- W2383359120 title "An Analysis of MMC-induced ASTC-a-1 Apoptosis in Living Cells" @default.
- W2383359120 hasPublicationYear "2008" @default.
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