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- W2383364288 abstract "[Objective] To study the antitumor effect of different extract parts of pine needles in vitro. [Methods] The extracts by pine needles were separated by different solvents. MTT was used to observe the inhibitory of extracts on the prolifera-tion of human hepatocellular carcinoma SMMC-7721, HepG2, breast carcinoma MCF-7 and prostatic carcinoma PC-3. Apoptosis rates and cell cycle distribution were detected by flow cytometry analysis. [Results] Petroleum ether extract from pine needles possessed the role of antitumor effect in cell culture. The inhibitory effect on the proliferation of tumor cells had remark-able time-dependence and dose-dependence. Petroleum ether extract was sensitivity to SMMC-7721. Petroleum ether extract induced SMMC-7721 apoptosis and cell cycle was arrested in G2-M phase. Apoptotic percentage of Petroleum ether extract 0.8mg· ml-1 on SMMC-7721 was 15.43%, 20.73% and 13.47% in 24 hours, 48 hours and 72 hours respectively. [Conclusion] The petroleum ether extract possesses the role of antitumor effect in vitro.Anticancer mechanism of petroleum ether extract on SMMC-7721 may be direct cytotoxic effect, inducing cell apoptosis and inhibiting cell segmentation." @default.
- W2383364288 created "2016-06-24" @default.
- W2383364288 creator A5082396860 @default.
- W2383364288 date "2009-01-01" @default.
- W2383364288 modified "2023-09-24" @default.
- W2383364288 title "STUDY ON ANTITUMOR EFFECT OF DIFFERENT EXTRACT PARTS OF PINE NEEDLES IN VITRO" @default.
- W2383364288 hasPublicationYear "2009" @default.
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