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- W2383390391 abstract "In order to acquire highly purified protein of cysteine domain of nonstructural protein 2 of PRRSV and acquaintance its antigen epiopes,the experiment were conducted with the genome extracted from PRRSV-VR2332 and got the gene of the cysteine domain of nonstructural protein 2 of PRRSV(Nsp2pro)by RT-PCR.Then,a recombinant plasmid named SUMO-Nsp2pro had been constructed,and then transformed SUMO-Nsp2pro into Rosetta2.Therefore,a large amount of Nsp2pro was obtained by IPTG.In the end,Nsp2pro was purified by His-Bind affinity chromatography,meanwhile predicted the second structure and antigen epiopes through means of bio-information,the results showed that Nsp2pro owned a great many Alpha regions and Beta regions while few of turn regions.The antigen epitopes were located in hydrolicity regions possibly.The obtained high purified protein would provide foundations for the further researches on Nsp2pro gene." @default.
- W2383390391 created "2016-06-24" @default.
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- W2383390391 date "2012-01-01" @default.
- W2383390391 modified "2023-09-23" @default.
- W2383390391 title "The Gene Clone,Prokaryotic Expression and Antigen Epitopes Prediction of the Cysteine Domain of Nonstructural Protein 2 of PRRSV" @default.
- W2383390391 hasPublicationYear "2012" @default.
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