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- W2383399900 abstract "Objective:To establish a novel suspension array technology for the detection of chloramphenicol(CAP) and clenbuterol(CL) residues. Methods: Two conjugates coupling the 2 veterinary drugs with BSA were synthesized and identified by UV spectrophotometry and mass spectrometry. Then, they were immobilized on the solid carrier of the suspension array-the polystyrene fluorescent microspheres/beads. There were competitive reactions between the veterinary drugs in the aqueous phase and that on the beads for combination with their specific biotinylated monoclonal antibodies. The amount of the veterinary drug conjugates and the antibodies were optimized and selected. The detective standard curves were plotted,and the specificity and the unknown samples were also determined. Results: Successful coupling was completed between small molecular veterinary drugs and BSA. The coupling ratio of CAP and CL with BSA was 40∶1 and 31∶1,respectively. The optimum amounts of the two conjugates were 5 μg per 100 μl beads and that of the two antibodies were 5 and 1ng per 2000 beads,respectively. The detective standard curves and their corresponding coefficients of determination were YCAP=-250.323+4103.517/[1+(X/30121.243)0.980], r2=0.994 and YCL=-263012.682+ 265751.765/[1+(X/6.063)0.115], r2=0.989. The detection ranges of the two veterinary drugs were 40-6.25×105ng/L and 50-7.81×105ng/L,respectively. The lowest detection limits of CAP and CL were below National Standards(GBs). Simultaneously, the specific detection of the suspension array was excellent and did not indicate significant cross-reactions with other drugs. Errors between the detected and the real concentration for the detection of the unknown samples were relatively small. Conclusion: The high-throughput suspension array provides a novel method for analysis of multiple veterinary drugs and has a good application,being user-friendly,highly sensitive,high-speed and cost-effective." @default.
- W2383399900 created "2016-06-24" @default.
- W2383399900 creator A5023591177 @default.
- W2383399900 date "2009-01-01" @default.
- W2383399900 modified "2023-09-25" @default.
- W2383399900 title "Detection of chloramphenicol and clenbuterol residues by high-throughput suspension array technology" @default.
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