Matches in SemOpenAlex for { <https://semopenalex.org/work/W2383424443> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2383424443 abstract "Obiective TO improve the plasmid vectors in gene therapy, adeno - associated virus (AAV) basedplasmid expressing vectors containing hIL - 2 gene or mIFN-γ gene were constructed and its expression intransfected cells was studied. Methods By means of step to step cloning, promoter CMVp was placed at thedownstream of 5' inverted terminal repeat from AAV (AAV - ITR) of pAP, hIL - 2 gene or mIFN -γ gene insertedinto pAC between CMVp and polyA. Then intron A was inserted into pAC - hIL - 2 or pAC- mIFN-γ betweenCMVp and IL - 2 gene or IFNγ gene to construct pAI- hIL - 2 or pAI- mIFN -γ. Liposome - plasmid complexeswere formed by mixing Dosper with these AAV- based plasmids containing hIL - 2 gene or mIFN- γgene. Results High biotogical activities of IL - 2 or IFN- γ could be detected in the supernatants of NIH3T3 andMM45T Li cells after transfection. Insertion of intron A into pAC- hIL - 2 or pAC- mIFN - γ improved theexpression of IL - 2 or IFN- γ. Conclusion These data demonstrated that the constructed AAV-based plasmidexpressing vectors could ejlciently express therapeutic genes in cultured cells and could be used as a nonviral genetransfer system in human gene therapy." @default.
- W2383424443 created "2016-06-24" @default.
- W2383424443 creator A5003570901 @default.
- W2383424443 creator A5013635257 @default.
- W2383424443 date "2000-01-01" @default.
- W2383424443 modified "2023-09-23" @default.
- W2383424443 title "CONSTRUCTION AND EXPRESSION OF ADENO-ASSOCIATED VIRUS-BASED PLASMID EXPRESSING VECTORS CONTAINING hIL-2 GENE OR mIFN-γ GENE" @default.
- W2383424443 hasPublicationYear "2000" @default.
- W2383424443 type Work @default.
- W2383424443 sameAs 2383424443 @default.
- W2383424443 citedByCount "0" @default.
- W2383424443 crossrefType "journal-article" @default.
- W2383424443 hasAuthorship W2383424443A5003570901 @default.
- W2383424443 hasAuthorship W2383424443A5013635257 @default.
- W2383424443 hasConcept C104317684 @default.
- W2383424443 hasConcept C111599444 @default.
- W2383424443 hasConcept C121050878 @default.
- W2383424443 hasConcept C150194340 @default.
- W2383424443 hasConcept C153911025 @default.
- W2383424443 hasConcept C159047783 @default.
- W2383424443 hasConcept C161733203 @default.
- W2383424443 hasConcept C199360897 @default.
- W2383424443 hasConcept C22744801 @default.
- W2383424443 hasConcept C2778107364 @default.
- W2383424443 hasConcept C40767141 @default.
- W2383424443 hasConcept C41008148 @default.
- W2383424443 hasConcept C54009773 @default.
- W2383424443 hasConcept C54355233 @default.
- W2383424443 hasConcept C86803240 @default.
- W2383424443 hasConcept C92087593 @default.
- W2383424443 hasConceptScore W2383424443C104317684 @default.
- W2383424443 hasConceptScore W2383424443C111599444 @default.
- W2383424443 hasConceptScore W2383424443C121050878 @default.
- W2383424443 hasConceptScore W2383424443C150194340 @default.
- W2383424443 hasConceptScore W2383424443C153911025 @default.
- W2383424443 hasConceptScore W2383424443C159047783 @default.
- W2383424443 hasConceptScore W2383424443C161733203 @default.
- W2383424443 hasConceptScore W2383424443C199360897 @default.
- W2383424443 hasConceptScore W2383424443C22744801 @default.
- W2383424443 hasConceptScore W2383424443C2778107364 @default.
- W2383424443 hasConceptScore W2383424443C40767141 @default.
- W2383424443 hasConceptScore W2383424443C41008148 @default.
- W2383424443 hasConceptScore W2383424443C54009773 @default.
- W2383424443 hasConceptScore W2383424443C54355233 @default.
- W2383424443 hasConceptScore W2383424443C86803240 @default.
- W2383424443 hasConceptScore W2383424443C92087593 @default.
- W2383424443 hasOpenAccess W2383424443 @default.
- W2383424443 hasRelatedWork W2112366226 @default.
- W2383424443 hasRelatedWork W2130862245 @default.
- W2383424443 hasRelatedWork W2355601149 @default.
- W2383424443 hasRelatedWork W2364827421 @default.
- W2383424443 hasRelatedWork W2365427361 @default.
- W2383424443 hasRelatedWork W2377849370 @default.
- W2383424443 hasRelatedWork W2378253534 @default.
- W2383424443 hasRelatedWork W2380102142 @default.
- W2383424443 hasRelatedWork W2389748941 @default.
- W2383424443 hasRelatedWork W2394262909 @default.
- W2383424443 hasRelatedWork W2397142423 @default.
- W2383424443 hasRelatedWork W2420122227 @default.
- W2383424443 hasRelatedWork W2421677230 @default.
- W2383424443 hasRelatedWork W2463907783 @default.
- W2383424443 hasRelatedWork W360951591 @default.
- W2383424443 hasRelatedWork W974688454 @default.
- W2383424443 hasRelatedWork W2188458189 @default.
- W2383424443 hasRelatedWork W2791459855 @default.
- W2383424443 hasRelatedWork W2829356148 @default.
- W2383424443 hasRelatedWork W2849452994 @default.
- W2383424443 isParatext "false" @default.
- W2383424443 isRetracted "false" @default.
- W2383424443 magId "2383424443" @default.
- W2383424443 workType "article" @default.