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- W2383483488 abstract "Objective To develop a simple,rapid,sensitive assay for the detection of heart-type fatty acid-binding protein(H-FABP) by using colloidal gold immunochromatography.Methods The H-FABP monoclonal antibody 67D3 was conjugated with colloid gold,and immobilized onto a dry membrane with biotinylated monoclonal antibody 66E2 together.Streptavidin was coupled with the nitrocellulose strip to prepare the test apparatus.The results were analyzed according to the red line on nitrocellulose membrane.The rapid one-step immunochromatography assay was constructed and used for the detection of plasma H-FABP from 93 patients with suspected AMI in 6 hours after onset of chest pain.The diagnostic validity was evaluated in terms of sensitivity and specificity compared with those of cardiac troponin-I(cTnI) and creatine kinase-MB (CK-MB).Results The lowest limit of the immunochromatographic assay for detection of H-FABP was 16.8 ng/mL.Compared with the results obtained by ELISA,the coincidence rate of the two assays was 96.9%.The sensitivity of H-FABP for AMI diagnosis was 64.29% within 3 hours and 84.38 % within 6 hours after onset of chest pain,which were significantly higher than those of cTnI(28.57% and 53.13%) and CK-MB (21.43% and 56.25%) respectively.There was no significant difference in specificity between the two assays.Conclusion The simple,rapid and sensitive immunochromatographic assay for detection of H-FABP may be applicable in the early diagnosis of AMI." @default.
- W2383483488 created "2016-06-24" @default.
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- W2383483488 date "2006-01-01" @default.
- W2383483488 modified "2023-09-24" @default.
- W2383483488 title "Development of a rapid test for heart-type fatty acid-binding protein (H-FABP)" @default.
- W2383483488 hasPublicationYear "2006" @default.
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