Matches in SemOpenAlex for { <https://semopenalex.org/work/W2383521412> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2383521412 abstract "To obtain a protein fragment of Streptococcus pneumoniae histidine kinase YycG with in vitro ATP-dependent kinase activity for screening novel bacterial inhibitors,the genomic DNA encoding the kinase domain of YycG was expressed in E.coli BL21(DE3).The product was identified by SDS-PAGE and Western blot assays,purified by a His-tagged Ni affinity column,and determined for kinase activity.It was about 35 kDa,reaching a purity of 95%,and has dose-dependent kinase activity of hydrolyzing ATP in vitro.Based on the inhibitory action on the catalyzing function of the purified kinase protein,several effective inhibitors were screened out from 105 candidate compounds available,one of which was selected out and verified overt bactericidal effects in vitro.Collectively,a fragment of histidine kinase YycG expressed in vitro was successful used to screen kinase inhibitors that may be important micro-molecule compound for developing antibacterial drugs or disinfectant." @default.
- W2383521412 created "2016-06-24" @default.
- W2383521412 creator A5088346840 @default.
- W2383521412 date "2009-01-01" @default.
- W2383521412 modified "2023-09-23" @default.
- W2383521412 title "Application of Histidine Kinase (YycG) in Screening Inhibitor of Streptococcus pneumoniae" @default.
- W2383521412 hasPublicationYear "2009" @default.
- W2383521412 type Work @default.
- W2383521412 sameAs 2383521412 @default.
- W2383521412 citedByCount "0" @default.
- W2383521412 crossrefType "journal-article" @default.
- W2383521412 hasAuthorship W2383521412A5088346840 @default.
- W2383521412 hasConcept C153911025 @default.
- W2383521412 hasConcept C181199279 @default.
- W2383521412 hasConcept C184235292 @default.
- W2383521412 hasConcept C185592680 @default.
- W2383521412 hasConcept C202751555 @default.
- W2383521412 hasConcept C2778460671 @default.
- W2383521412 hasConcept C2778905257 @default.
- W2383521412 hasConcept C2781253189 @default.
- W2383521412 hasConcept C501593827 @default.
- W2383521412 hasConcept C55493867 @default.
- W2383521412 hasConcept C86803240 @default.
- W2383521412 hasConcept C97029542 @default.
- W2383521412 hasConceptScore W2383521412C153911025 @default.
- W2383521412 hasConceptScore W2383521412C181199279 @default.
- W2383521412 hasConceptScore W2383521412C184235292 @default.
- W2383521412 hasConceptScore W2383521412C185592680 @default.
- W2383521412 hasConceptScore W2383521412C202751555 @default.
- W2383521412 hasConceptScore W2383521412C2778460671 @default.
- W2383521412 hasConceptScore W2383521412C2778905257 @default.
- W2383521412 hasConceptScore W2383521412C2781253189 @default.
- W2383521412 hasConceptScore W2383521412C501593827 @default.
- W2383521412 hasConceptScore W2383521412C55493867 @default.
- W2383521412 hasConceptScore W2383521412C86803240 @default.
- W2383521412 hasConceptScore W2383521412C97029542 @default.
- W2383521412 hasOpenAccess W2383521412 @default.
- W2383521412 hasRelatedWork W1566042048 @default.
- W2383521412 hasRelatedWork W1986373657 @default.
- W2383521412 hasRelatedWork W2008083853 @default.
- W2383521412 hasRelatedWork W2072292761 @default.
- W2383521412 hasRelatedWork W2093237042 @default.
- W2383521412 hasRelatedWork W2105860371 @default.
- W2383521412 hasRelatedWork W2108092697 @default.
- W2383521412 hasRelatedWork W2110923541 @default.
- W2383521412 hasRelatedWork W2136971046 @default.
- W2383521412 hasRelatedWork W2186632447 @default.
- W2383521412 hasRelatedWork W2290999512 @default.
- W2383521412 hasRelatedWork W2292282034 @default.
- W2383521412 hasRelatedWork W2399136570 @default.
- W2383521412 hasRelatedWork W2415026910 @default.
- W2383521412 hasRelatedWork W2416905262 @default.
- W2383521412 hasRelatedWork W2793327475 @default.
- W2383521412 hasRelatedWork W2804595444 @default.
- W2383521412 hasRelatedWork W3149984777 @default.
- W2383521412 hasRelatedWork W2290774444 @default.
- W2383521412 hasRelatedWork W2821607527 @default.
- W2383521412 isParatext "false" @default.
- W2383521412 isRetracted "false" @default.
- W2383521412 magId "2383521412" @default.
- W2383521412 workType "article" @default.