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- W2383527412 abstract "Extraction of high-quality RNA from the phyllode of Acacia crassicarpa is very difficult due to high levels of tannin,phenols,pigment,cellulose polysaccharide that bind or coprecipitate with RNA.There-fore,the preferred RNA isolation protocols were optimized from the phyllode of Acacia crassicarpa,compar-ing the Trizol method,improved hot-borate methods,CTAB and RNA isolation kit combined with ultrasonic method.It was found that the RNA isolation kit combined with 20 MHz ultrasonic processor(puls on 5 s,puls off 5 s) was optimal for the RNA isolation from Acacia crassicarpa.The extracted total RNA with this method had high integrity,the ratio of A260/A280 was in the range of 1.82～1.98,and had been successfully used for RT-PCR experiment." @default.
- W2383527412 created "2016-06-24" @default.
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- W2383527412 date "2012-01-01" @default.
- W2383527412 modified "2023-09-28" @default.
- W2383527412 title "The Establishment and Optimization of RNA Isolation in Acacia crassicarpa" @default.
- W2383527412 hasPublicationYear "2012" @default.
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