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- W2383551805 abstract "This study was conducted to prepare for comparison of the prokaryotic and eukaryotic EG95-(C3d)3proteins in the immune effects and for development of efficient method of detection Echinococcosis by building recombinant baculovirus containing three copies of C3dgene and EG95s gene of Echinococcus granulosus.EG95s gene of and three tandem copies of goat C3dgene were inserted to pTarget vector to obtain recombinant plasmid,named pTarget-EG95-(C3d)3,then the target gene,EG95-(C3d)3,were cloned into the pFastBac-Hta of Bac-to-Bac system by BamH I and XbaI,the recombinant plasmid,pFastBacHta-EG95-(C3d)3,were obtained and then was transformed into the DH10 Bac for transposon reorganization.The recombinant transposon,rBacmid-EG95-(C3d)3,were transfected into Sf9insect cells for obtain the EG95-(C3d)3 recombinant baculovirus and expression of EG95-(C3d)3 recombinant proteins.The proteins were identified by SDS-PAGE and Western blotting.rUsing the recombinant proteins detected sheep antibodies against Echinococcosis granulosa.The results showed that the EG95-(C3d)3 recombinant baculovirus was obtained,and the EG95-(C3d)3recombinant protein with the size of about 132ku were correctly expressed in insect cells in Sf9,the results of Western blotting showed that this EG95-(C3d)3 protein could response to Echinococcus granulosus-specific positive serum,indicating that EG95-(C3d)3protein has immune activity.The recombinant proteins had well sensitivity with Echinococcosis granulosa.The EG95-(C3d)3 gene was correctly expressed in insect cells,which built the bases for comparison of the immune effects of prokaryotic and eukaryotic EG95-(C3d)3 proteins,and for development of efficient method of detection Echinococcosis." @default.
- W2383551805 created "2016-06-24" @default.
- W2383551805 creator A5062857320 @default.
- W2383551805 date "2010-01-01" @default.
- W2383551805 modified "2023-09-26" @default.
- W2383551805 title "Tandem Expression and Identification of EG95s Gene of Echinococcus granulosus and C3d Gene Using Baculovirus Expression System" @default.
- W2383551805 hasPublicationYear "2010" @default.
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