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- W2383553474 abstract "Objective To explore the treatment of human breast cancer by adeno-associated virus (AAV) expressing HSVtk gene under the regulation of Tet regulatory system, the recombinant AAV-2 were transduced into MCF-7 cell line. Methods The Tet-On and HSVtk gene was respectively inserted into the AAV cassettes(pAAV MCS),then rAAV/HSVtk/Tet-On were generated by co-transfection 293 cells with other plasmids(pAAV-RC、pHelper). The viral titer of rAAV/HSVtk/Tet-On and its expression were measured by β-galactosidase staining, dot blot and RT-PCR methods after purification and concentration of rAAV. These viruses were then direct transduced into MCF-7 cells. Results The results of dot blot indicate that the rAAV can infect MCF-7 cells and transfer target gene into host cells. The expression of HSVtk gene in MCF-7 cells was obeserved by RT-PCR analysis. The antiproliferation effect on infected MCF-7 cells was detected by MTT assay. It was showed that the infected MCF-7 cells with GCV treatment under the doxycycline(Dox) induction had greater inhibition effect than the control induction when viral particle number ≥104v.p(viral particl)/cell. Conclusion The results demonstrated that recombinant AAV containing Tet regulation system and HSVtk gene were successfully transferred into MCF-7 target cells." @default.
- W2383553474 created "2016-06-24" @default.
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- W2383553474 date "2007-01-01" @default.
- W2383553474 modified "2023-09-23" @default.
- W2383553474 title "Gene Transfection Efficiency and Expression of Regulatory Recombinant Adeno-Associated Virus Gene Transfer through Direct Infection on MCF-7 Cells" @default.
- W2383553474 hasPublicationYear "2007" @default.
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