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- W2383583051 abstract "In order to establish the optimized ISSR-PCR experiment system for Pogostemon cablin,the single factor experiments were used to detect the suitable concentration ranges of the different influential factors,and then the orthogonal design was performed to optimize 5 factors(Mg2+,dNTPs,primer,Taq polymerase,DNA template) at 4 levels,which affect ISSR-PCR amplification system of Pogostemon cablin.The results indicated that a suitable ISSR-PCR reaction system established: 10× buffer 2.5 μL,40 ng DNA template,2.5 mmol·L-1 MgCl2,0.3 μmol·L-1 primers,1.5 U Taq polymerase,150 μmol·L-1 dNTPs were contained in 25 μL reaction solution.The optimized amplification program was that predenaturing at 94 ℃ for 5 min,then denaturing at 94 ℃ for 45 s,primer annealing at 52.7 ℃ for 45 s,extension at 72 ℃ for 90 s,for 40 cycles,at last extension at 72 ℃ for 7 min,and the products were stored at 4 ℃." @default.
- W2383583051 created "2016-06-24" @default.
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- W2383583051 date "2011-01-01" @default.
- W2383583051 modified "2023-09-24" @default.
- W2383583051 title "Optimization of ISSR-PCR Experimental System for Pogostemon cablin with the Dual Experiment Methods of the Single Factor and the Orthogonal Design" @default.
- W2383583051 hasPublicationYear "2011" @default.
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