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- W2383587652 abstract "One cold-adapted lactase producing strain was isolated from Tiansan mount permafrost in the Xinjiang Uygur Autonomous Region.Based on its characteristics of morphology,cultivation,physiology and biochemistry and 16S rDNA sequence alignment,this strain was identified as Rahnella sp.and named Rahnella sp.R3.The optimal growth temperature of the strain and the optimal temperature for producing enzyme were both 15℃.Under the optimal fermentation conditions,the maximum enzyme activity was 5.92 U/mL.The lactase was intracellular enzyme.Research on its enzymatic properties showed that the lactase was maximally active at 25℃ and pH 7.0-8.0.And it had higher enzyme activity at low temperature,91%at 15℃ and 40%at 4℃,respectively.The enzyme activity was thermal-sensitive and was undetectable after 1 h incubated at 65℃.Some metal ions had inhibiting effects on enzyme activity.Ca~(2+) and Na~+ slightly reduced enzyme activity while heavy metal ions,particularly Cu~(2+),Al~(3+) and Zn~(2+) strongly inhibited enzyme activity." @default.
- W2383587652 created "2016-06-24" @default.
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- W2383587652 date "2013-01-01" @default.
- W2383587652 modified "2023-09-26" @default.
- W2383587652 title "Screening,identification of Rahnella sp.R3 producing cold-adapted lactase and its enzymatic properties" @default.
- W2383587652 hasPublicationYear "2013" @default.
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